Tag: DNA fingerprinting

The Rediscovery Of The Colorado Orange Apple: NPR

The Rediscovery Of The Colorado Orange Apple

In Colorado they have rediscovered a long-lost apple, the Colorado Orange apple. Jude Schuenemeyer talks about the discovery.

 
December 29, 20197:54 AM ET
Heard on Weekend Edition Sunday

  

 Copyright 2019 NPR. To see more, visit https://www.npr.org.
LULU GARCIA-NAVARRO, HOST:As the decade comes to a close, we can now report that, in fact, you can mix apples and oranges – well, kind of. In the western United States, apple anthropologists are excited about the rediscovery of an apple variety that was believed to be extinct. It’s called the Colorado Orange apple. Jude Schuenemeyer of the Montezuma Orchard Restoration Project in southwestern Colorado helped track down the orange apple. We asked him what makes it so special.

JUDE SCHUENEMEYER: So the Colorado Orange is a mix of sweet and tart. Being a winter apple, the flavor opens up over time. So winter apples, like the Colorado Orange, you wouldn’t even think of starting to eat until Christmas. You’d go through all your summer apples, all your fall apples. You’d have these in the root cellar. And then starting Christmas, you’d start to pull these out. And month by month, the flavor would open up. A little bit of the sweet would go – a little bit of the tang might. But they were still going to be very flavorful. They have some of the most complex flavors of any apples you’ll ever have. Culinary-wise, they have some of the most complex flavors you’ll ever have in anything.

GARCIA-NAVARRO: The Colorado Orange was popular in the late 1800s. But around the 1940s, it started to disappear.

SCHUENEMEYER: The biggest thing it had going against it was that it was a yellowish-orange-ish-glowed apple at a time when America was going into monoculture where shiny, red apples were considered the only apples worth buying. It wasn’t because it was bad quality or didn’t grow well. It lost out, like so many of the thousands and thousands of apple varieties that have gone extinct. It lost out because it wasn’t the shiny, red apple.

GARCIA-NAVARRO: Schuenemeyer says locals around Canyon City, Colo., cherished the orange apple.

SCHUENEMEYER: They knew it was a really high-quality apple, winner apple, good keeping apple. And in Canyon City, the memory of it was kept alive for a long time. And the old timer’s like, oh, yeah. I used to have a tree. It died. But we kept thinking there was still going to be one around. We felt like we could still find one.

GARCIA-NAVARRO: And so he and his wife, Addie, started combing the state.

SCHUENEMEYER: Two years ago, we were in Canyon City in this orchard in December. And this person, Mr. Diana, said, hey. I’ve got a tree also. And he took us to a tree. My wife Addie and I looked at it. And lo and behold, on the ground underneath the tree in the duff, there were these orange-blushed apples. And then on the tree, there were some of the apples still hanging. And it had that really good, sub-acid, flavorful taste that you’d expect from a winter Apple. So yeah. It was a a big moment for us.

GARCIA-NAVARRO: But the Shuenemeyers have been careful and taking their time. They did cutting-edge DNA testing and compared their find to some archival wax apple replicas at Colorado State University. They wanted to make sure they’d found the actual orange apple of memory.

SCHUENEMEYER: Because it’s considered extinct, there’s probably never an absolute. But we’ve got as close to an absolute as we can. Between this newest new DNA technology, the historical purveyors of the orchard itself and the waxed apple to compare it to, that’s an extraordinary amount of information that most people would never be able to have to compare anything.

GARCIA-NAVARRO: And so in a couple of years, once the young trees get going, keep your eyes open in the produce section for something new. And please restrain yourself from asking Jude Schuenemeyer about mixing apples and oranges.

SCHUENEMEYER: (Laughter) Yeah, yeah. People – when they hear of the Colorado orange, they definitely wonder what we’re talking about. That’s for sure.

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Lost for decades, the Colorado Orange apple variety has been found — officially

The Montezuma Orchard Restoration Project compared the fruit of a tree found near Cañon City to botanical illustrations and wax castings of award-winning apples to identify the lost treasure. Read the full article in The Colorado Sun. DEC 18, 2019 5:07AM MST

Nancy Lofholm

Special to The Colorado Sun

Also, read the story of the elusive Colorado Orange apple in MORP’s own words.

An apple revival near Four Corners is restoring hundreds of historic fruits — and the local ag economy

Using DNA testing in southwest Colorado, the Montezuma Orchard Restoration project welcomes back apple varieties like Winter Banana, Blue Pearmain, Ben Davis and Esopus Spitzenburg — and businesses are sprouting around them.

MCELMO CANYON — The apple orchard on Jude and Addie Schuenemeyer’s farm in a squiggle of a canyon in far southwest Colorado is a wild place. Turkeys gobble around on the hunt for bugs in native grasses that grow nearly as high as the gnarly limbs of the apple trees. Those trees are set hither and thither instead of lining up in typical tidy orchard rows. They bear apples that few fruit fans have likely heard of: Winter Banana, Blue Pearmain, Ben Davis and Esopus Spitzenburg…read the full article in The Colorado Sun.

Nancy Lofholm 
PUBLISHED ON NOV 28, 2019 5:05AM MST

MORP DNA Results of Historic Apple Trees

MORP DNA results of 489 apple leaf samples collected by MORP and submitted to the USDA-ARS National Laboratory for Genetic Resource Preservation for identification, sorted by name. Click the arrows at the bottom of the document to scroll through all pages or click the link to see full document. MORP DNA Results_name http://montezumaorchard.org/wp-content/uploads/2017/11/MORP-DNA-Results_name.pdf

MORP DNA results of 489 apple leaf samples sorted by tree ID number. Click the arrows at the bottom of the document to scroll through all pages or click the link to see full document. MORP DNA results_treeID http://montezumaorchard.org/wp-content/uploads/2017/11/MORP-DNA-results_treeID.pdf 

Details:

  • 58 named cultivars
  • 34 unknown cultivar matches to other samples – likely named cultivars not in ARS dataset
  • 103 unique unknown cultivars – some are likely seedlings. However, MORP took care to collect from grafted – not seedling trees – so many of these unique unknowns are also likely named historic cultivars not listed in the ARS dataset
  • 195 cultivars in total out of 489 MORP samples

Testing made possible by a 2015 Colorado USDA Specialty Crop Block Grant award to MORP

Historic Apple Cultivar Identification Using DNA Fingerprinting Techniques by Gayle Volk, USDA-ARS National Laboratory for Genetic Resource Preservation (article from MORP 2016 newsletter) 

Apple cultivars are traditionally vegetatively propagated by grafting; many apple cultivars have been sold and exchanged over the centuries. During the American homestead era, apple trees were planted on properties as part of the process of cultivating the land. Cultivars purchased as grafted trees from nurseries often had desirable traits, such as large, higher quality fruit that could be eaten fresh, stored for extended lengths of time, or used for cider production. Trees planted from seeds often did not exhibit desirable traits for fresh consumption, and were instead used primarily for cider. Many historic apple cultivars remain available today as grafted trees in national and private collections. In fact, DNA genetic fingerprinting techniques have been used to develop a database of fingerprints of materials in the USDA collection for use in unknown cultivar identification.

An informal collaboration among the Montezuma Orchard Restoration Project, historic orchards of Wyoming (both through USDA Specialty Crop Research Grants), Yosemite and Redwood National Parks, as well as El Dorado National Forest is underway to identify locally important historic apple cultivars. This effort seeks to use known historic cultivars in the USDA- ARS National Plant Germplasm System Apple Collection—as well as selected varieties in collections at Washington State University and the Temperate Orchard Conservancy (Oregon)—as standards to determine the identities of unknown apples.

Leaf tissue from key historic apple trees was sent to the National Laboratory for Genetic Resources Preservation in Fort Collins, Colorado. A graduate student from the University of Wyoming has been extracting DNA from these leaf samples and will be preparing the extracts for fingerprinting analyses. Molecular markers, termed “microsatellites”, will be used to compare the genetic identities of the unknown (or tentatively named) cultivars to those in known collections. We hope to be able to identify many of the grafted materials that were previously unknown. This method of genetic testing will only yield cultivar names for grafted varieties; therefore, historic trees that originated from seedling sources will likely remain unidentified.

Publications that relate to this work are: One is a publication by Kanin Routson, Ann Reilley, Adam Henk and Gayle Volk titled “Identification of Historic Apple Trees in the Southwestern United States and Implications for Conservation” (HortScience 2009. 44:589-594) and another was  published by Gayle Volk and Adam Henk “Historic American Apple Cultivars: Identication and Availability” (J. Amer. Soc. Hort. Sci. 2016. 141:292-301).